E-ISSN 2790-2218
 

Research Article

Online Publishing Date:
24 / 05 / 2023



Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue

Sabikun Nahar, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, Marzia Rahman.


Abstract
Objective: This study was outlined for the isolation and identification of some zoonotic bacte¬ria present in broiler meat, along with the investigation of antimicrobial resistance patterns and determination of antibiotic residue.
Materials and Methods: 45 raw meat samples were collected from different restaurants in Mymensingh. Staphylococcus spp., Escherichia coli, Salmonella spp., Proteus spp., and Citrobacter spp. were detected through cultural, morphological, and biochemical tests. The E. coli were identified by amplification of malB gene, and Staphylococcus aureus was identified by targeting 16r- RNA gene. The disc diffusion method was applied for the antibiogram investigation. Thin layer chromatography was performed to determine the antibiotic residue within the broiler meat.
Results: Staphylococcus spp., E. coli, Salmonella spp., Proteus spp., and Citrobacter spp. were detected with a prevalence of 93.33% (42/45), 35.56% (16/45), 2.22% (1/45), 8.89% (4/45), and 13.33% (6/45), respectively. Antibiogram study revealed that Staphylococcus spp. showed the highest resistance to Trimethoprim and Oxytetracycline (75%), and the highest sensitivity was observed toward Gentamycin. The antibiogram profile of E. coli revealed 100% resistance to Oxytetracycline and 100% sensitivity to Gentamycin. Salmonella spp. showed resistance to Ciprofloxacin, Enrofloxacin, and Levofloxacin and was sensitive to Florfenicol and Gentamycin. The antibiotic profile of Proteus spp. exhibited 66.7% resistance to Trimethoprim, Ciprofloxacin, and Enrofloxacin. Citrobacter spp. showed 100% resistance to Trimethoprim, 66.7% resistance to Levofloxacin, and 100% sensitivity was observed to Florfenicol, Gentamycin, and Norfloxacin. The antibiotic residue of Ciprofloxacin, Levofloxacin, Oxytetracycline, and Enrofloxacin was also determined. In the case of antibiotic residue determination, the residue of Levofloxacin, Oxytetracycline, and Ciprofloxacin was found to be 6.67%, 6.67%, and 13.33%, respectively.
Conclusion: This study revealed the existence of some multidrug-resistant pathogenic bacteria and higher limits of antibiotic residue in broiler meat and liver which may affect human health.

Key words: Antimicrobial resistance; Staphylococcus spp.; E. coli; Salmonella spp.; Proteus; Citrobacter; antibiotic residue


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Sabikun Nahar
Articles by Md. Arefin Kallol
Articles by Khudaza Akter Lima
Articles by Masuma Aktar Bristy
Articles by Zannatul Haque Tumpa
Articles by Jayedul Hassan
Articles by K. H. M. Nazmul Hussain Nazir
Articles by Marzia Rahman
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Nahar S, Kallol MA, Lima KA, Bristy MA, Tumpa ZH, Hassan J, , Rahman M. Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. Vet. Res. Notes. 2023; 3(5): 43-49. doi:10.5455/vrn.2023.c27


Web Style

Nahar S, Kallol MA, Lima KA, Bristy MA, Tumpa ZH, Hassan J, , Rahman M. Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. https://www.vetresnotes.com/?mno=127709 [Access: March 06, 2024]. doi:10.5455/vrn.2023.c27


AMA (American Medical Association) Style

Nahar S, Kallol MA, Lima KA, Bristy MA, Tumpa ZH, Hassan J, , Rahman M. Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. Vet. Res. Notes. 2023; 3(5): 43-49. doi:10.5455/vrn.2023.c27



Vancouver/ICMJE Style

Nahar S, Kallol MA, Lima KA, Bristy MA, Tumpa ZH, Hassan J, , Rahman M. Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. Vet. Res. Notes. (2023), [cited March 06, 2024]; 3(5): 43-49. doi:10.5455/vrn.2023.c27



Harvard Style

Nahar, S., Kallol, . M. A., Lima, . K. A., Bristy, . M. A., Tumpa, . Z. H., Hassan, . J., & Rahman, . M. (2023) Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. Vet. Res. Notes, 3 (5), 43-49. doi:10.5455/vrn.2023.c27



Turabian Style

Nahar, Sabikun, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, and Marzia Rahman. 2023. Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. Veterinary Research Notes, 3 (5), 43-49. doi:10.5455/vrn.2023.c27



Chicago Style

Nahar, Sabikun, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, and Marzia Rahman. "Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue." Veterinary Research Notes 3 (2023), 43-49. doi:10.5455/vrn.2023.c27



MLA (The Modern Language Association) Style

Nahar, Sabikun, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, and Marzia Rahman. "Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue." Veterinary Research Notes 3.5 (2023), 43-49. Print. doi:10.5455/vrn.2023.c27



APA (American Psychological Association) Style

Nahar, S., Kallol, . M. A., Lima, . K. A., Bristy, . M. A., Tumpa, . Z. H., Hassan, . J., & Rahman, . M. (2023) Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue. Veterinary Research Notes, 3 (5), 43-49. doi:10.5455/vrn.2023.c27





Most Viewed Articles
Most Accessed Articles

  • Isolation and molecular characterization of Shiga-toxin producing Escherichia coli from betel leaf (Piper betel L.)
    Jafrul Hasan Ripon, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Shanta Das, Md. Bahanur Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 12-16
    » Abstract » doi: 10.5455/vrn.2021.a3

  • Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health
    Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(1): 1-5
    » Abstract » doi: 10.5455/vrn.2021.a1

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2

  • Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
    Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 17-22
    » Abstract » doi: 10.5455/vrn.2021.a4

  • Dietary manipulation to improve the gut health of poultry
    Khan Md. Shaiful Islam, Mst. Mahomudha Akhtar
    Vet. Res. Notes. 2022; 2(1): 1-10
    » Abstract » doi: 10.5455/vrn.2022.b6

  • Most Downloaded
    Top Downloaded Articles

  • Dietary manipulation to improve the gut health of poultry
    Khan Md. Shaiful Islam, Mst. Mahomudha Akhtar
    Vet. Res. Notes. 2022; 2(1): 1-10
    » Abstract » doi: 10.5455/vrn.2022.b6

  • Possible use of seaweed (Gracilaria tenuistipitata Var. Liui) to the reduction of enteric methane emissions from dairy cattle
    Md. Shahjahan Ali Sarker, Md. Shakil Anwar, Hasan Zohirul Islam, Md. Rezaul Alam, Hasin Ishraq Reefat
    Vet. Res. Notes. 2022; 2(11): 78-85
    » Abstract » doi: 10.5455/vrn.2022.b18

  • Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue
    Sabikun Nahar, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, Marzia Rahman
    Vet. Res. Notes. 2023; 3(5): 43-49
    » Abstract » doi: 10.5455/vrn.2023.c27

  • Evaluation of the effectiveness of phytogenic feed additive on hatchable egg production performance in Cobb 430 Y breeders under field conditions
    Vishwanath Gopal Bhagwat, Balamurugan Ellusamy, Rangesh Paramesh
    Vet. Res. Notes. 2021; 1(3): 23-26
    » Abstract » doi: 10.5455/vrn.2021.a5

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2

  • Most Cited Articles
    Most Cited Articles

  • Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
    Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 17-22
    » Abstract » doi: 10.5455/vrn.2021.a4
    Cited : 1 time [Click to see citing article]

  • Disease prevalence and use of veterinary antibiotics in land-based aquaculture in South Chattogram, Bangladesh: A matter of health concern
    Md. Abu Kawsar, Nishat Tasnim, Fatema Jannat Munny
    Vet. Res. Notes. 2022; 2(2): 15-21
    » Abstract » doi: 10.5455/vrn.2022.b8
    Cited : 1 time [Click to see citing article]

  • Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health
    Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(1): 1-5
    » Abstract » doi: 10.5455/vrn.2021.a1
    Cited : 0 time [Click to see citing article]

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2
    Cited : 0 time [Click to see citing article]

  • Isolation and molecular characterization of Shiga-toxin producing Escherichia coli from betel leaf (Piper betel L.)
    Jafrul Hasan Ripon, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Shanta Das, Md. Bahanur Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 12-16
    » Abstract » doi: 10.5455/vrn.2021.a3
    Cited : 0 time [Click to see citing article]