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Research Article


Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health

Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir.

Abstract
Objectives: The purpose of this study was to isolate and identify Candida spp. and Saccharomyces spp. from bakery products (bread and cake) using a range of cultural (e.g., colony shape, size, color, elevation, surface edge, consistency, etc.) and morphological (e.g., cell shape, bud, etc.) features.
Materials and Methods: Cake and bread samples (n = 40) were obtained from various retail out¬lets in Bangladesh Agricultural University (BAU), Mymensingh. The samples were collected asep¬tically and transferred to the Department of Microbiology and Hygiene at BAU, Mymensingh, for analysis. Processed samples were inoculated onto Potato Dextrose Agar media and incubated at a humidity of 75% for 5-7 days at 28°C. The fungi were primarily identified based on their colony structures. DNA was extracted from suspected colonies and was used for the detection of Candida spp. and Saccharomyces spp. using genus-specific primers (ITS-1 and ITS-4).
Results: Candida spp. and Saccharomyces spp. had a combined prevalence of 50% (n = 20/40; p < 0.05). Candida spp. was identified in 15% (n = 6/40) of the fungi, while Saccharomyces spp. was detected in 35% (n = 14/40).
Conclusion: In conclusion, it can be stated that bakery foods have a significant impact on human fungal infection in Bangladesh.

Key words: Candida spp.; Bakery foods; PCR; Saccharomyces spp.


 
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How to Cite this Article
Pubmed Style

Khanam S, Shahid MAH, Mahmud MM, Hossain MZ, Khatun MM, Nazir KNH. Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. Vet. Res. Notes. 2021; 1(1): 1-5. doi:10.5455/vrn.2021.a1


Web Style

Khanam S, Shahid MAH, Mahmud MM, Hossain MZ, Khatun MM, Nazir KNH. Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. https://www.vetresnotes.com/?mno=117864 [Access: August 16, 2023]. doi:10.5455/vrn.2021.a1


AMA (American Medical Association) Style

Khanam S, Shahid MAH, Mahmud MM, Hossain MZ, Khatun MM, Nazir KNH. Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. Vet. Res. Notes. 2021; 1(1): 1-5. doi:10.5455/vrn.2021.a1



Vancouver/ICMJE Style

Khanam S, Shahid MAH, Mahmud MM, Hossain MZ, Khatun MM, Nazir KNH. Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. Vet. Res. Notes. (2021), [cited August 16, 2023]; 1(1): 1-5. doi:10.5455/vrn.2021.a1



Harvard Style

Khanam, S., Shahid, . M. A. H., Mahmud, . M. M., Hossain, . M. Z., Khatun, . M. M. & Nazir, . K. N. H. (2021) Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. Vet. Res. Notes, 1 (1), 1-5. doi:10.5455/vrn.2021.a1



Turabian Style

Khanam, Shurya, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, and KHM Nazmul Hussain Nazir. 2021. Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. Veterinary Research Notes, 1 (1), 1-5. doi:10.5455/vrn.2021.a1



Chicago Style

Khanam, Shurya, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, and KHM Nazmul Hussain Nazir. "Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health." Veterinary Research Notes 1 (2021), 1-5. doi:10.5455/vrn.2021.a1



MLA (The Modern Language Association) Style

Khanam, Shurya, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, and KHM Nazmul Hussain Nazir. "Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health." Veterinary Research Notes 1.1 (2021), 1-5. Print. doi:10.5455/vrn.2021.a1



APA (American Psychological Association) Style

Khanam, S., Shahid, . M. A. H., Mahmud, . M. M., Hossain, . M. Z., Khatun, . M. M. & Nazir, . K. N. H. (2021) Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health. Veterinary Research Notes, 1 (1), 1-5. doi:10.5455/vrn.2021.a1





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