E-ISSN 2790-2218
 

Research Article

Online Publishing Date:
08 / 11 / 2021



Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj

S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir.


Abstract
Objectives: E. coli O157 is considered as one of the important pathogens causing subclinical mastitis in dairy cows. This study was undertaken to isolate E. coli O157 from the milk samples collected from subclinical mastitic cows using polymerase chain reaction (PCR) and investigate their antibiotic sensitivity patterns.
Materials and Methods: 50 California Mastitis Test (CMT) positive milk samples were collected from apparently healthy crossbreed dairy cows in Baghabari, Sirajganj. For the enrichment and isolation of the organism, nutrient broth, MacConkey agar, and EMB agar were used. Later on, observing the biochemical tests result, all the isolates of E. coli were confirmed by PCR using genus-specific 16SrRNA primers. PCR-positive samples were then screened for the presence of the rfbO157 gene using gene-specific primers. The antibiotic sensitivity pattern of E. coli was assessed by the disk diffusion method against seven commonly used antibiotics.
Results: Altogether, 8 (16%) isolates of E. coli were obtained, among which 5 (10%) were rfbO157 PCR positive. From the antibiotic sensitivity test, Gentamicin was the highest (75%) sensitive to the isolates, followed by Levofloxacin (62.5%), Cefixime (50%), Tetracycline (50%), and Ceftriaxone (25%). The highest resistance pattern was found against Ampicillin (100%) and Amoxycillin (87.5%).
Conclusion: Raw milk containing E. coli O157 does not only reflect the status of the dairy herd. Additionally, it poses a serious threat to human health if it is consumed raw or used to make any type of value-added food product.

Key words: Subclinical mastitis; E. coli O157; Prevalence; PCR; Antibiogram


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by S. M. Farhad
Articles by Md. Ahosanul Haque Shahid
Articles by Md. Muket Mahmud
Articles by Ajran Kabir
Articles by Shanta Das
Articles by Md. Tanvir Rahman
Articles by KHM Nazmul Hussain Nazir
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Farhad SM, Shahid MAH, Mahmud MM, Kabir A, Das S, Rahman MT, Nazir KNH. Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. Vet. Res. Notes. 2021; 1(2): 6-11. doi:10.5455/vrn.2021.a2


Web Style

Farhad SM, Shahid MAH, Mahmud MM, Kabir A, Das S, Rahman MT, Nazir KNH. Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. https://www.vetresnotes.com/?mno=117862 [Access: July 10, 2024]. doi:10.5455/vrn.2021.a2


AMA (American Medical Association) Style

Farhad SM, Shahid MAH, Mahmud MM, Kabir A, Das S, Rahman MT, Nazir KNH. Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. Vet. Res. Notes. 2021; 1(2): 6-11. doi:10.5455/vrn.2021.a2



Vancouver/ICMJE Style

Farhad SM, Shahid MAH, Mahmud MM, Kabir A, Das S, Rahman MT, Nazir KNH. Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. Vet. Res. Notes. (2021), [cited July 10, 2024]; 1(2): 6-11. doi:10.5455/vrn.2021.a2



Harvard Style

Farhad, S. M., Shahid, . M. A. H., Mahmud, . M. M., Kabir, . A., Das, . S., Rahman, . M. T. & Nazir, . K. N. H. (2021) Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. Vet. Res. Notes, 1 (2), 6-11. doi:10.5455/vrn.2021.a2



Turabian Style

Farhad, S. M., Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, and KHM Nazmul Hussain Nazir. 2021. Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. Veterinary Research Notes, 1 (2), 6-11. doi:10.5455/vrn.2021.a2



Chicago Style

Farhad, S. M., Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, and KHM Nazmul Hussain Nazir. "Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj." Veterinary Research Notes 1 (2021), 6-11. doi:10.5455/vrn.2021.a2



MLA (The Modern Language Association) Style

Farhad, S. M., Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, and KHM Nazmul Hussain Nazir. "Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj." Veterinary Research Notes 1.2 (2021), 6-11. Print. doi:10.5455/vrn.2021.a2



APA (American Psychological Association) Style

Farhad, S. M., Shahid, . M. A. H., Mahmud, . M. M., Kabir, . A., Das, . S., Rahman, . M. T. & Nazir, . K. N. H. (2021) Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj. Veterinary Research Notes, 1 (2), 6-11. doi:10.5455/vrn.2021.a2





Most Viewed Articles
Most Accessed Articles

  • Isolation and molecular characterization of Shiga-toxin producing Escherichia coli from betel leaf (Piper betel L.)
    Jafrul Hasan Ripon, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Shanta Das, Md. Bahanur Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 12-16
    » Abstract » doi: 10.5455/vrn.2021.a3

  • Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health
    Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(1): 1-5
    » Abstract » doi: 10.5455/vrn.2021.a1

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2

  • Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
    Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 17-22
    » Abstract » doi: 10.5455/vrn.2021.a4

  • Dietary manipulation to improve the gut health of poultry
    Khan Md. Shaiful Islam, Mst. Mahomudha Akhtar
    Vet. Res. Notes. 2022; 2(1): 1-10
    » Abstract » doi: 10.5455/vrn.2022.b6

  • Most Downloaded
    Top Downloaded Articles

  • Dietary manipulation to improve the gut health of poultry
    Khan Md. Shaiful Islam, Mst. Mahomudha Akhtar
    Vet. Res. Notes. 2022; 2(1): 1-10
    » Abstract » doi: 10.5455/vrn.2022.b6

  • Possible use of seaweed (Gracilaria tenuistipitata Var. Liui) to the reduction of enteric methane emissions from dairy cattle
    Md. Shahjahan Ali Sarker, Md. Shakil Anwar, Hasan Zohirul Islam, Md. Rezaul Alam, Hasin Ishraq Reefat
    Vet. Res. Notes. 2022; 2(11): 78-85
    » Abstract » doi: 10.5455/vrn.2022.b18

  • Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue
    Sabikun Nahar, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, Marzia Rahman
    Vet. Res. Notes. 2023; 3(5): 43-49
    » Abstract » doi: 10.5455/vrn.2023.c27

  • Evaluation of the effectiveness of phytogenic feed additive on hatchable egg production performance in Cobb 430 Y breeders under field conditions
    Vishwanath Gopal Bhagwat, Balamurugan Ellusamy, Rangesh Paramesh
    Vet. Res. Notes. 2021; 1(3): 23-26
    » Abstract » doi: 10.5455/vrn.2021.a5

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2

  • Most Cited Articles
    Most Cited Articles

  • Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
    Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 17-22
    » Abstract » doi: 10.5455/vrn.2021.a4
    Cited : 1 time [Click to see citing article]

  • Disease prevalence and use of veterinary antibiotics in land-based aquaculture in South Chattogram, Bangladesh: A matter of health concern
    Md. Abu Kawsar, Nishat Tasnim, Fatema Jannat Munny
    Vet. Res. Notes. 2022; 2(2): 15-21
    » Abstract » doi: 10.5455/vrn.2022.b8
    Cited : 1 time [Click to see citing article]

  • Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health
    Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(1): 1-5
    » Abstract » doi: 10.5455/vrn.2021.a1
    Cited : 0 time [Click to see citing article]

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2
    Cited : 0 time [Click to see citing article]

  • Isolation and molecular characterization of Shiga-toxin producing Escherichia coli from betel leaf (Piper betel L.)
    Jafrul Hasan Ripon, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Shanta Das, Md. Bahanur Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 12-16
    » Abstract » doi: 10.5455/vrn.2021.a3
    Cited : 0 time [Click to see citing article]