E-ISSN 2790-2218
 
2024, Vol: 4, Issue: 10
4 / 10Current Issue Online First Archive Aims and Scope Abstracting & Indexing Most Accessed Articles Most Downloaded Articles Most Cited Articles

Research Article
Online Published: 14 Nov 2021


Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms

Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir.


Cited By:1

Abstract
Objectives: The goal of this study was to isolate, molecularly identify, and antibiogram of Staphylococcus aureus, as well as to investigate risk factors for subclinical mastitis in the Jhenaidah and Chuadanga districts of Bangladesh.
Materials and Methods: A total of 100 milk samples were collected from apparently healthy goats at various farms in the Jhenaidah and Chuadanga districts. To detect samples with subclinical mastitis (SCM), the California Mastitis Test (CMT) was done. S. aureus was isolated and identified from milk samples that tested positive for CMT using a combination of standard bacteriological examinations, biochemical tests, and PCR confirmation. The disc diffusion test was used to determine the antibiogram status of the isolates. The risk factors for SCM in goats were analyzed using a semi-structured questionnaire with thirteen variables.
Results: Some characteristics were significantly associated with goat mastitis, including farm, breed, shed construction, floor surface condition, and farm adviser. The CMT identified 52 positive samples for SCM, with 73.07% (n = 38/52) suggesting the presence of the nuc gene. Antibiotic susceptibility testing revealed that the isolated S. aureus was totally resistant to the antibiotics Penicillin, Oxacillin, and Vancomycin (21%). On the other hand, Tetracycline, Gentamycin, Norfloxacin, and Levofloxacin were all susceptible to all isolates.
Conclusion: SCM is a severe problem in goats in Jhenaidah and Chuadanga districts. To minimize the risk of SCM infection in goats, hygiene precautions can be followed, and education among goat farm owners and farmers can be improved.

Key words: Antibiogram; nuc gene; PCR; Subclinical mastitis; S. aureus.


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Md. Abu Zihad
Articles by Md. Ahosanul Haque Shahid
Articles by Md. Muket Mahmud
Articles by Ajran Kabir
Articles by Md. Shahriar Kamal
Articles by Jannatun Naim
Articles by Md. Liakot Hossen
Articles by KHM Nazmul Hussain Nazir
on Google
on Google Scholar


How to Cite this Article
Pubmed Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes. 2021; 1(2): 17-22. doi:10.5455/vrn.2021.a4

Web Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. https://www.vetresnotes.com/?mno=116426 [Access: November 27, 2024]. doi:10.5455/vrn.2021.a4

AMA (American Medical Association) Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes. 2021; 1(2): 17-22. doi:10.5455/vrn.2021.a4


Vancouver/ICMJE Style

Zihad MA, Shahid MAH, Mahmud MM, Kabir A, Kamal MS, Naim J, Hossen ML, Nazir KNH. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes. (2021), [cited November 27, 2024]; 1(2): 17-22. doi:10.5455/vrn.2021.a4


Harvard Style

Zihad, M. A., Shahid, . M. A. H., Mahmud, . M. M., Kabir, . A., Kamal, . M. S., Naim, . J., Hossen, . M. L. & Nazir, . K. N. H. (2021) Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Vet. Res. Notes, 1 (2), 17-22. doi:10.5455/vrn.2021.a4


Turabian Style

Zihad, Md. Abu, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, and KHM Nazmul Hussain Nazir. 2021. Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Veterinary Research Notes, 1 (2), 17-22. doi:10.5455/vrn.2021.a4


Chicago Style

Zihad, Md. Abu, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, and KHM Nazmul Hussain Nazir. "Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms." Veterinary Research Notes 1 (2021), 17-22. doi:10.5455/vrn.2021.a4


MLA (The Modern Language Association) Style

Zihad, Md. Abu, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, and KHM Nazmul Hussain Nazir. "Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms." Veterinary Research Notes 1.2 (2021), 17-22. Print. doi:10.5455/vrn.2021.a4


APA (American Psychological Association) Style

Zihad, M. A., Shahid, . M. A. H., Mahmud, . M. M., Kabir, . A., Kamal, . M. S., Naim, . J., Hossen, . M. L. & Nazir, . K. N. H. (2021) Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms. Veterinary Research Notes, 1 (2), 17-22. doi:10.5455/vrn.2021.a4




Most Viewed Articles
Most Accessed Articles

  • Isolation and molecular characterization of Shiga-toxin producing Escherichia coli from betel leaf (Piper betel L.)
    Jafrul Hasan Ripon, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Shanta Das, Md. Bahanur Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 12-16
    » Abstract » doi: 10.5455/vrn.2021.a3

  • Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health
    Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(1): 1-5
    » Abstract » doi: 10.5455/vrn.2021.a1

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2

  • Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
    Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 17-22
    » Abstract » doi: 10.5455/vrn.2021.a4

  • Dietary manipulation to improve the gut health of poultry
    Khan Md. Shaiful Islam, Mst. Mahomudha Akhtar
    Vet. Res. Notes. 2022; 2(1): 1-10
    » Abstract » doi: 10.5455/vrn.2022.b6

    •
    Most Downloaded
    Top Downloaded Articles

  • Dietary manipulation to improve the gut health of poultry
    Khan Md. Shaiful Islam, Mst. Mahomudha Akhtar
    Vet. Res. Notes. 2022; 2(1): 1-10
    » Abstract » doi: 10.5455/vrn.2022.b6

  • Possible use of seaweed (Gracilaria tenuistipitata Var. Liui) to the reduction of enteric methane emissions from dairy cattle
    Md. Shahjahan Ali Sarker, Md. Shakil Anwar, Hasan Zohirul Islam, Md. Rezaul Alam, Hasin Ishraq Reefat
    Vet. Res. Notes. 2022; 2(11): 78-85
    » Abstract » doi: 10.5455/vrn.2022.b18

  • Antimicrobial resistance pattern of bacteria isolated from broiler meat with the determination of antibiotic residue
    Sabikun Nahar, Md. Arefin Kallol, Khudaza Akter Lima, Masuma Aktar Bristy, Zannatul Haque Tumpa, Jayedul Hassan, K. H. M. Nazmul Hussain Nazir, Marzia Rahman
    Vet. Res. Notes. 2023; 3(5): 43-49
    » Abstract » doi: 10.5455/vrn.2023.c27

  • Evaluation of the effectiveness of phytogenic feed additive on hatchable egg production performance in Cobb 430 Y breeders under field conditions
    Vishwanath Gopal Bhagwat, Balamurugan Ellusamy, Rangesh Paramesh
    Vet. Res. Notes. 2021; 1(3): 23-26
    » Abstract » doi: 10.5455/vrn.2021.a5

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2

    •
    Most Cited Articles
    Most Cited Articles

  • Molecular detection, antibiogram, and risk factor analysis of Staphylococcus aureus from subclinical mastitis of goats in conventional and organized farms
    Md. Abu Zihad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Md. Shahriar Kamal, Jannatun Naim, Md. Liakot Hossen, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 17-22
    » Abstract » doi: 10.5455/vrn.2021.a4
    Cited : 1 time [Click to see citing article]

  • Disease prevalence and use of veterinary antibiotics in land-based aquaculture in South Chattogram, Bangladesh: A matter of health concern
    Md. Abu Kawsar, Nishat Tasnim, Fatema Jannat Munny
    Vet. Res. Notes. 2022; 2(2): 15-21
    » Abstract » doi: 10.5455/vrn.2022.b8
    Cited : 1 time [Click to see citing article]

  • Isolation and molecular identification of Candida spp. and Saccharomyces spp. from bakery products and their impact on public health
    Shurya Khanam, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Md. Zawad Hossain, Mst. Minara Khatun, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(1): 1-5
    » Abstract » doi: 10.5455/vrn.2021.a1
    Cited : 0 time [Click to see citing article]

  • Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj
    S. M. Farhad, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Ajran Kabir, Shanta Das, Md. Tanvir Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 6-11
    » Abstract » doi: 10.5455/vrn.2021.a2
    Cited : 0 time [Click to see citing article]

  • Isolation and molecular characterization of Shiga-toxin producing Escherichia coli from betel leaf (Piper betel L.)
    Jafrul Hasan Ripon, Md. Ahosanul Haque Shahid, Md. Muket Mahmud, Shanta Das, Md. Bahanur Rahman, KHM Nazmul Hussain Nazir
    Vet. Res. Notes. 2021; 1(2): 12-16
    » Abstract » doi: 10.5455/vrn.2021.a3
    Cited : 0 time [Click to see citing article]

    •

    About Veterinary Research Notes

    Veterinary Research Notes (VRN) is an open access, international, peer-reviewed, monthly scientific journal publishing original research findings and re ... Read more.



    For best results, please use Internet Explorer or Google Chrome.

    Contact Information

    Network for the Veterinarians of Bangladesh (BDvetNET), Prof. Nazmul H. Nazir, DVM, MS, PhD, Dept. of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, BANGLADESH. E-mail: bdvetnet@gmail.com or nazir@bau.edu.bd
    POLICIES & JOURNAL LINKS
    Author Login
    Reviewer Login
    About Publisher
    Advertising Policy
    Author's Rights and Obligations
    Conflict of Interest Policy
    Copyright Information
    Digital Archiving & Preservation Policies
    Editorial Policies
    Peer Review Policy
    Editorial & Peer Review Process
    License Information
    Open Access Policy
    Plagiarism Policy
    Privacy Policy
    Protection of Research Participants (Statement On Human And Animal Rights)
    Publication Ethics and Publication Malpractice Statement
    Corrections, Retractions & Expressions of Concern
    Self-Archiving Policies
    Statement of Informed Consent
    Terms of Use